• Researchers have developed the world’s first species-agnostic nanosensor that enables non-destructive, real-time monitoring of plants’ primary growth hormone: a form of auxin called indole-3-acetic acid (IAA)
• Auxins help plants to regulate their development, and stress responses such as shade or high temperature – making it a key indicator of plant health
• Using the corona phase molecular recognition (CoPhMoRe) technique, the nanosensor can precisely track IAA levels in different crop types without the need for genetic modification
• This breakthrough technology holds significant potential for agricultural applications, allowing farmers to monitor plant growth and stress response, and develop stress-tolerant crops

Researchers from the Disruptive & Sustainable Technologies for Agricultural Precision (DiSTAP) interdisciplinary research group (IRG) of Singapore-MIT Alliance for Research and Technology (SMART), MIT’s research enterprise in Singapore, in collaboration with Temasek Life Sciences Laboratory (TLL) and Massachusetts Institute of Technology (MIT), have developed the world’s first near-infrared (NIR) fluorescent nanosensor capable of real-time, non-destructive and species-agnostic detection of indole-3-acetic acid (IAA) – the primary bioactive auxin hormone that controls the way plants develop, grow and respond to stress.

Auxins, particularly IAA, play a central role in regulating key plant processes such as cell division, elongation, root and shoot development, and response to environmental cues like light, heat, and drought. External factors like light affect how auxin moves within the plant, temperature influences how much is produced, and a lack of water can disrupt hormone balance. When plants cannot effectively regulate auxins, they may not grow well, adapt to changing conditions, or produce as much food. 

Existing IAA detection methods, such as liquid chromatography, require taking plant samples from the plant – which harms or removes part of it. Conventional methods also measure the effects of IAA rather than detecting it directly, and cannot be used universally across different plant types. In addition, since IAA is a small molecule that cannot be easily tracked in real-time, biosensors that contain fluorescent proteins need to be inserted into the plant’s genome to measure auxin, making it emit a fluorescent signal for live imaging.

SMART’s newly developed nanosensor enables direct, real-time tracking of auxin levels in living plants with high precision. The sensor uses NIR imaging to monitor IAA fluctuations non-invasively across tissues like leaves, roots, and cotyledons, and it is capable of bypassing chlorophyll interference to ensure highly reliable readings even in densely pigmented tissues. The technology does not require genetic modification and can be integrated with existing agricultural systems – offering a scalable precision tool to advance both crop optimisation and fundamental plant physiology research. 

By providing real-time, precise measurements of auxin – a hormone central to plant growth and stress response – the sensor empowers farmers with earlier and more accurate insights into plant health. With these insights and comprehensive data, farmers can make smarter, data-driven decisions on irrigation, nutrient delivery, and pruning, tailored to the plant’s actual needs – ultimately improving crop growth, boosting stress resilienc,e and increasing yields.

“We need new technologies to address the problems of food insecurity and climate change worldwide. Auxin is a central growth signal within living plants, and this work gives us a way to tap it to give new information to farmers and researchers. The applications are many, including early detection of plant stress, allowing for timely interventions to safeguard crops. For urban and indoor farms, where light, water and nutrients are already tightly controlled, this sensor can be a valuable tool in fine-tuning growth conditions with even greater precision to optimise yield and sustainability,” saidProf Michael Strano, Co-Lead Principal Investigator at DiSTAP and Carbon P. Dubbs Professor of Chemical Engineering at MIT, and co-corresponding author of the paper. 

The research team documented the nanosensor’s development in a paper, titled “A Near-Infrared Fluorescent Nanosensor for Direct and Real-Time Measurement of Indole-3-Acetic Acid in Plants”, published in the journal ACS Nano. The sensor comprises single-walled carbon nanotubes (SWNTs) wrapped in a specially designed polymer, which enables it to detect IAA through changes in NIR fluorescence intensity. Successfully tested across multiple species, including Arabidopsis, Nicotiana benthamiana, choy sum, and spinach, the nanosensor can map IAA responses under various environmental conditions such as shade, low light, and heat stress. 

“This sensor builds on DiSTAP’s ongoing work in nanotechnology and the CoPhMoRe technique, which has already been used to develop other sensors that can detect important plant compounds such as gibberellins and hydrogen peroxide. By adapting this approach for IAA, we’re adding to our inventory of novel, precise and non-destructive tools for monitoring plant health. Eventually, these sensors can be multiplexed, or combined, to monitor a spectrum of plant growth markers for more complete insights into plant physiology,” said Dr Duc Thinh Khong, Principal Research Scientist at DiSTAP and co-first author of the paper.

“This small but mighty nanosensor tackles a long-standing challenge in agriculture: the need for a universal, real-time and non-invasive tool to monitor plant health across various species. Our collaborative achievement not only empowers researchers and farmers to optimise growth conditions and improve crop yield and resilience, but also advances our scientific understanding of hormone pathways and plant-environment interactions,” said Dr In-Cheol Jang, Senior Principal Investigator at TLL and Principal Investigator at DiSTAP, and co-corresponding author of the paper.

Looking ahead, the research team is looking to combine multiple sensing platforms to simultaneously detect IAA and its related metabolites to create a comprehensive hormone signaling profile, offering deeper insights into plant stress responses and enhancing precision agriculture. They are also working on using microneedles for highly localised, tissue-specific sensing and collaborating with industrial urban farming partners to translate the technology into practical, field-ready solutions. 

The research is carried out by SMART and supported by the National Research Foundation under its Campus for Research Excellence and Technological Enterprise (CREATE) programme.