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Stickers and a smartphone for easy nitrite detection on foods

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Stickers and a smartphone for easy nitrite detection on foods

August 8, 2022 by Asia Food Journal

nitrite detection

Courtesy of ACS Applied Materials & Interfaces 2022

Nitrates and nitrites give processed meats their characteristic pink colour and robust flavour. Although many consumers want to limit consumption of these preservatives because the substances can form potentially cancer-causing compounds, knowing how much is in food has been difficult to determine. Now, researchers reporting in ACS Applied Materials & Interfaces have developed a colour-changing film that consumers can stick onto foods and easily analyse nitrite levels by snapping a picture with a smartphone.

Cured and processed meats, such as salami and bacon, are often treated with nitrite or nitrate salts to keep them looking and tasting fresh. Though nitrate is relatively stable, it can be converted to the more reactive nitrite ion in the body. When in the acidic environment of the stomach or under the high heat of a frying pan, nitrite can undergo a reaction to form nitrosamines, which have been linked to the development of various cancers. Some methods to determine nitrite levels in foods already exist, but they are not very consumer-friendly and often require expensive and laborious techniques and instruments. To help consumers make more informed decisions, Saúl Vallejos, José M. García, and colleagues wanted to develop an easy-to-use nitrite quantification system.

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To accomplish this, the researchers developed a film called “POLYSEN,” which stands for “polymeric sensor,” made of four monomers and hydrochloric acid. Disks punched from the material were placed on meat samples for 15 minutes, allowing the monomer units and acid in the film to react with nitrite in a four-step azo coupling reaction. The disks were then removed and dipped in a sodium hydroxide solution for one minute to develop the colour. When nitrite was present, the film’s yellowish hue deepened with higher nitrite levels in the food. To quantitate the colour change, the researchers created a smartphone app that self-calibrates when a chart of reference disks is photographed in the same image as the sample disks.

The team tested the film on meats they prepared and treated with nitrite, in addition to store-bought meats, and found that the POLYSEN-based method produced results similar to those obtained with a traditional and more complex nitrite detection method. In addition, POLYSEN complied with European regulations for the migration of substances from the film to the food. The researchers say the new approach could be a user-friendly and inexpensive way for consumers to determine nitrite levels in foods.

The authors acknowledge funding from La Caixa Foundation and the Spanish Agencia Estatal de Investigación. They also acknowledge the support and collaboration of the company Inforapps.

Other Topics: ACS, food processing, food processing technology, Nitrite, Polysen, Processing, technology

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