DairyX is successfully crafting next-gen casein micelles using precision fermentation

YAVNE, ISRAEL – DairyX Foods Ltd. announces a major advancement in creating authentic milk proteins without cows, using precision fermentation. The food-tech start-up has developed a method to produce casein proteins that can self-assemble into micelles. Micelles are the primary building blocks of dairy products, such as cheese and yogurt.  

DairyX has also refined a complementary technology to enhance the gelation of its casein micelles, considered the holy grail of the industry. DairyX’s gelating micelles enable manufacturers to produce firm, stretchy and creamy products using their traditional dairy-making processes.

Creating a smarter casein with precision fermentation 

Consumers can’t resist dairy’s taste and robust health benefits. Casein micelles are key to the appealing sensory profile of dairy products. 

DairyX’s precision fermentation technology uses microorganisms (specifically yeast) to produce smart casein proteins. “Not all caseins produced using precision fermentation are alike,” explains Maya Bar-Zeev, PhD, Head of Product Development and Downstream Processing. “We trained yeast to produce the next generation of casein. DairyX’s patent-pending casein is an advanced form created to precisely and effectively organize into micelles.” 

“The industry knows quite well that caseins are extremely hard to produce using precision fermentation, so our initial goal was to solve this problem. Once we successfully crafted caseins, the next major challenge was to upgrade caseins so they could self-assemble into gelating micelles to produce the dairy properties manufacturers are seeking,” explains DairyX CEO and founder Arik Ryvkin, PhD. 

Currently, manufacturers of animal-free dairy products use additives, like stabilizers, emulsifiers and thickeners, which don’t perform as well as cow’s milk and can add unpleasant aftertastes. These fail to satisfy consumer cravings for a real dairy experience.

“DairyX caseins have amino acid sequences identical to those of their animal counterparts, making them, in fact, non-genetically modified,” explains Galit Kuznets, Head of Strain Development and Fermentation. “Our casein also eliminates the need for hormones and antibiotics applied in dairy farms. 

Maximized efficiency

For consumers, taste and price are dairy’s two most important features. DairyX addresses taste with its innovative caseins while also making non-animal dairy affordable. The company is creating yeast strains that produce exceptionally high casein yields in short timeframes. This approach ensures that DairyX’s ingredients are cost-effective – a crucial factor for adoption by dairy manufacturers. 

“Another significant challenge that dairy companies face is adapting their production facilities to use new ingredients,” Bar-Zeev explains. “This is why we created a drop-in replacement for milk that does not require process changes or retooling.”

The future of dairy proteins begins here

“I am a fan of cows—just not for producing dairy,” says Ryvkin. “As a scientist and former vegan, I didn’t eat plant-based dairy for a decade because I didn’t find truly tasty and additive-free products. To genuinely mimic traditional dairy, producers needed to start with different source ingredients.” Dr. Ryvkin had the vision and the scientific expertise to create sustainable dairy proteins that are identical to cow’s milk proteins in structure and function. Supported by a team of biotechnology and industry experts, he forged ahead with the pioneering development of these next-generation dairy ingredients.

Worldwide, 270 million cows spend their lives in the production of dairy products. Not only does this impact the cows, it also harms the environment. Dairy production is responsible for over 3% of global greenhouse gas emissions and uses excessive farmland and water. Totally eliminating cow’s milk as a food source is not practical, so DairyX is devoted to lowering the dairy industry’s dependence on traditional milk as a raw ingredient. Doing so promotes sustainability, improved animal welfare, and the use of precious land and water for better causes.

As protein ingredients, DairyX micelles have no cholesterol or lactose, giving our clients, the CPG companies, the versatility to decide which fats and sugars to add to their formulations.”

DairyX deep tech makes all the difference

Kuznets discusses DairyX’s proprietary technologies and protocols, saying, “We have achieved several key objectives with our solutions. Our biological design genetically manipulated yeast to produce functional caseins that we organized into micelles. We developed a fast-tracked screening process that simulated evolution to locate super-producers of protein from among millions of yeast strains. Our machine-learning models simulated fermentation to determine optimal fermentation conditions. We have proven our ability to create a gel from reconstituted casein micelles. All these ingenuities have helped us work smarter and faster to create highly functional micelles.”

On the horizon

“Recreating casein protein micelles from yeast is an important milestone,” concludes Ryvkin. “We are currently focused on scaling up our fermentation process and collaborating with dairy companies. These achievements bring us one essential step closer to helping dairy companies make the exact products consumers desire while helping cows live happier lives.”

About DairyX

Founded in 2022, DairyX is on a mission to replace cows as sole dairy protein sources through the use of precision fermentation. The company develops animal-free dairy proteins for food companies to produce tasty, nutritious products that are easy to manufacture. Topping the DairyX agenda is a commitment to climate resilience and reduced greenhouse gas emissions and resource use. DairyX solutions are created by a talented multidisciplinary group of scientists, fermentation experts, product developers, and advisors who are pioneers in their fields. DairyX investors include: Peregrine Ventures, Jesselson, Phibro Animal Health Corporation, the Israel Innovation Authority and Incentive – Peregrine’s Incubator. The company has signed a letter of intent with CSM Ingredients and several other dairy partners.